Engineering Of with Lipases

Engineering of/with Lipases presents two major topics: the design and production of lipases with desired, preselected properties, and the use of lipases for desired applications.

Engineering Of with Lipases

The innovative uses of lipases in a wide variety of organic syntheses and the modification of existing fats and oils have increased exponentially over the last five years, due to the increasing availability of lipases from (genetically engineered) microbial sources, coupled with their special capacity to act as catalysts at hydrophilic/hydrophobic interfaces. As a result of the structural characterisation performed during the same period, applications of these lipases can now be developed in a much more rational way. Engineering of/with Lipases presents two major topics: the design and production of lipases with desired, preselected properties, and the use of lipases for desired applications. Audience: Doctoral and post-doctoral crystallographers, biochemists, geneticists and enzyme kineticists. Food, chemical and biochemical engineers. The former will learn about the practical aims and constraints associated with industrial applications of lipases, enabling them to design lipases for specific purposes. The latter will learn how to take advantage of the structural knowledge of lipases and their metabolic genesis to better design media, processes and products in terms of biochemical and technical feasibility.

Lipases

The 70 articles in the book, written by scientists from 16 nations (notably from Europe, USA and Japan), are grouped into four sections: * 3-Dimensional Structure * Mechanism of Action * Genetic Engineering * General Characterization and ...

Lipases

Lipases are of high practical relevance. They exhibit activity only at the water-lipid interphase and can thus be utilized for various industrial applications such as detergents and chiral synthesis. Up to now, however, progress in industrial use has been hampered by the lack of fundamental knowledge about lipase structure and function. With the information this GBF monograph provides, scientists will finally be able to develop and exploit lipases in their own work. The 70 articles in the book, written by scientists from 16 nations (notably from Europe, USA and Japan), are grouped into four sections: * 3-Dimensional Structure * Mechanism of Action * Genetic Engineering * General Characterization and Purification This unique and invaluable documentation is the result of a CEC-GBF Workshop (Braunschweig, Germany, September 1990) held within the framework of the BRIDGE EEC project.

Protein Engineering For Industrial Biotechnology

This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a section on its use to enhance purification of recombinant proteins.

Protein Engineering For Industrial Biotechnology

Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a section on its use to enhance purification of recombinant proteins. The use of protein engineering to modify the activity or the stability of industrial enzymes from lipases to proteases, from carboxypeptidases to glucanases and glucosidases, and from pectin modifying enzymes to enzymes able to degrade recalcitrant compounds is extensively covered. It is shown how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors receive significant contributions from protein and solvent engineering. The application of protein engineering to health care is also covered, from the development of new vaccines to new potential therapeutic proteins. A specific notation is given to protein engineering in the development of target molecules for drug discovery. International in scope, the many contributions are drawn from academia and industry. The text should be of interest to students and researchers in industrial biotechnology as well as to everybody interested in basic research in protein structure, molecular genetics, bio-organic chemistry, biochemistry, agrobiotechnology, pharmaceutical sciences and medicine.

Understanding Enzymes

Understanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the ...

Understanding Enzymes

Understanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the simulation work and nanotechnology measurement of enzymes in action in vitro or in silico. The book also presents new insights into the mechanistic function and understanding of enzyme reactions, as well as touching upon structural characteristics, including X-ray and nuclear magnetic resonance (NMR) structural methods. A major focus of the book is enzyme molecules’ dependency on dynamic and biophysical environmental impacts on their function in ensembles as well as single molecules. A wide range of readers, including academics, professionals, PhD and master’s students, industry experts, and chemists, will immensely benefit from this exclusive book.

Supercritical Fluids Technology in Lipase Catalyzed Processes

2.7.1 MEDIUM ENGINEERING The reaction medium can be improved by
modifying the medium polarity. This determines the solubility and stability of
lipase substrates, and therefore, lipase activity and selectivity. Introducing
organic solvents ...

Supercritical Fluids Technology in Lipase Catalyzed Processes

Enzymes are currently used in various industries, most commonly in food, detergents, and pharmaceuticals production. Lipases are hydrolytic enzymes that demonstrate great potential as an alternative to conventional catalysts in a number of industrial applications. A complete understanding of enzymes, and their proteins structure and environmental behavior, can greatly aid in the further development of industrial applications. Supercritical Fluids Technology in Lipase Catalized Processes provides basic information about enzymes, their sources, reaction kinetics, and main industrial applications. The book focuses in lipases. their main sources, structure, and features, with an emphasis on their specificity and interfacial activity, and presents proven techniques for isolating, extracting, and purifying. Comprised of six compact chapters, this comprehensive guide introduces: Immobilization techniques and immobilized lipases that allow repeated use (which is essential from an economic point of view) Different bioreactor configurations using immobilized lipases The latest information on the available technologies in lipolytic reactions The advantages of nonaqueous media in biochemical synthesis over aqueous and solvent-free systems Material on the use of lipases in nonaqueous media to overcome the drawbacks usually encountered with the use of conventional chemical catalysts The use of supercritical fluids (SCFs) as a green alternative reaction medium Factors affecting the physical properties of lipases in this medium and, hence, their activity and stability A case study using supercritical carbon dioxide (SC-CO2) for biodiesel production Novel, cutting-edge technology, using immobilized enzymes to reduce the overall production cost Supercritical Fluids Technology in Lipase Catalized Processes outlines the main industrial applications of common enzymes and discusses relevant challenges and innovations emerging in the field.

Chemical and Mechanical Engineering Information Technologies

It was reported that about 65 genus from microorganism produce lipase. In the
Lipase Engineering Database (LED) (http://www.led.uni- stuttgart.de), sequences
of 92 microbial lipases and homologous serine hydrolases were assigned to 32 ...

Chemical and Mechanical Engineering  Information Technologies

Collection of selected, peer reviewed papers from the 2013 3rd International Symposium on Chemical Engineering and Material Properties (ISCEMP 2013), June 22-24, 2013, Sanya, China. The 508 papers are grouped as follows: Chapter 1: Chemical Engineering and Technology, Bio and Medical Chemistry Engineering; Chapter 2: Material Science, Manufacturing Technology and Civil Engineering; Chapter 3: Mechanical Engineering and Equipment, Mechatronics, Automation and Control; Chapter 4: Measurement and Instrumentation, Monitoring, Testing and Detection Technologies, Fault Diagnosis; Chapter 5: Computation Methods and Algorithms for Modeling, Simulation and Optimization, Data Mining and Data Processing; Chapter 6: Information Technologies, WEB and Networks Engineering, Information Security, Software Application and Development; Chapter 7: Power and Energy, Electric and Magnetic Systems, Electronics and Microelectronics, Embedded and Integrated Systems; Chapter 8: Communication, Signal and Image Processing, Data Acquisition, Identification and Recognation Technologies; Chapter 9: Information Technologies in Management, Logistics, Economics, Finance and Assessment.

Computer and Information Science Applications in Bioprocess Engineering

MEMBRANE BIOREACTOR WITH IMMOBILIZED LIPASE : MODELLING AND
COMPUTATIONAL CONSIDERATIONS F. XAVIER MALCATA Escola ...
Computer and Information Science Applications in Bioprocess Engineering , 201-
220 .

Computer and Information Science Applications in Bioprocess Engineering

Proceedings of the NATO Advanced Study Institute on `Use of Computer and Informatic Systems in Bioprocess Engineering', Ofir, Portugal, May 18-29, 1992

Enzyme Biocatalysis

Lipases can be improved by tuning their properties according to their particular
applications by engineering either the biocatalyst and/or the reaction medium.
6.3.3.1 Medium Engineering Lipases catalyze the hydrolytic cleavage of ...

Enzyme Biocatalysis

This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.

Applied Biocatalysis

1.2 Enzyme Activity of Lipases from Different Sources and Engineering
Approaches So far, several microbial lipases have been purified, characterized,
and evaluated for their potentials in biotechnological applications. Bacterial
lipases ...

Applied Biocatalysis

This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.

Lipases Part A Biotechnology

[ 19 ] Protein Engineering of Microbial Lipases of Industrial Interest By ALLAN
SVENDSEN , IB GROTH CLAUSEN , SHAMKANT ANANT PATKAR , KIM
BORCH , and MARIANNE THELLERSEN Introduction Protein engineering of
industrial ...

Lipases  Part A  Biotechnology

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Handbook of Industrial Biocatalysis

Protein. Engineering. of. Recombinant. Candida. rugosa. Lipases. Guan-Chiun
Lee 6.1 6.2 6.3 6.4 Chwen-Jen Shieh Introduction ........................................................
............... 6-1 Differential Expression of CRL Isoforms ......................... 6-2 Structures
 ...

Handbook of Industrial Biocatalysis

Until now, no comprehensive handbook on industrial biocatalysis has been available. Soliciting chapters on virtually every aspect of biocatalysis from international experts most actively researching the field, the Handbook of Industrial Biocatalysis fills this need. The handbook is divided into three sections based on types of substrates. T

Enzyme Engineering XIV

Here, we will outline our approach to create a toolbox of recombinant,
overexpressed lipases of different origin in combination with a lipase database
allowing us to choose the most appropriate enzyme for a specific reaction, and to
engineer it ...

Enzyme Engineering XIV

These are the proceedings of an Engineering Foundation conference cosponsored by the Natural Science Foundation of China and the Chinese Society for Enzyme Engineering, October 12-17, 1997.

Lipid Biotechnology

This text presents the latest advances in supercritical fluid technology, biocatalysis, bioprocess engineering, and crop breeding.

Lipid Biotechnology

This text presents advances in supercritical fluid technology, biocatalysis, bioprocess engineering, and crop breeding. It offers an in-depth review of principles and approaches utilized in the development and design of lipids for cosmetic, industrial, pharmaceutical and food products.

Protein Engineering Handbook

The exact pathway that the slow enantiomer takes to react most likely differs for
different substrates and for different lipases. The steady-state kinetics for
enantioselective lipase-catalyzed resolutions fit either of the enantioselectivity ...

Protein Engineering Handbook

Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.

Journal of Chemical Engineering of Japan

g - Lipase ] Conversion [ % ] V . ( mmol / h Enzyme activity of surfactantlipase
complex 5 10 15 20 3 Reaction system Fig . 2 Fig . 3 Comparison of esterification
achieved by different reaction systems ( 1 , native lipase ; 2 , PEG - lipase
complex ...

Journal of Chemical Engineering of Japan

Includes abstracts of Kagaku kōgaku, v. 31-

Enzyme Engineering XII

The present crystal structure shows that the pancreatic lipase lid is a complicated
structural device . Upon lipid binding ... The importance of the lid in mammalian
lipases has recently been illustrated by an elegant study on lipoprotein lipase .

Enzyme Engineering XII

This text focuses on scientific as well as engineering aspects of enzyme technology, including recent advances in protein and metabolic engineering; supramolecular biochemistry (nanotechnology); new biocatalysts, such as ribozymes and abazymes; metal-based biocatalysts; and glycosylation. The important lipase programme of the EEC is widely discussed. The book presents a cross-fertilisation between microscopic (chemistry, molecular biology) and macroscopic (bioreactors, biosensors) levels of inquiry, revealing a complementarity between industrial, medical and analytical applications. Enzyme technology inheres in many facets of human activity: agriculture, pharmaceuticals, diagnostics, cosmetics, chemicals, pollution control and energy.

Enzyme Functionality

Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and ...

Enzyme Functionality

Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolution to accelerate the development of novel enzyme functionalities. This reference gathers the

Enzyme Engineering XIII

13 Two distinct lipase forms were obtained from CrL by Phenyl Sepharose
hydrophobic interaction and DEAE ... Both purified lipases were able to catalyze
the esterification of 1 - butanol and oleic acid and the transesterification of 2 -
ethyl ...

Enzyme Engineering XIII

This work looks at the interdisciplinary nature of the field of enzyme engineering, which includes the use of enzymes in biotechnology, synthetic chemistry, pharmaceutical development, and agriculture. Both fundamental and applied topics are covered, with each paper providing sufficient background to familiarise readers with the problems - and their potential solutions - that face those using enzymes for industrial applications.

Handbook of Molecular Microbial Ecology II

Because the catalytic reactions usually exhibit high chemo-, regio-, and/or
stereoselectivities, esterases and lipases ... Online databases like the Lipase
Engineering database (LED, http://www.led.uni-stuttgart.de), the Microbial
Esterase and ...

Handbook of Molecular Microbial Ecology II

The premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the first comprehensive two-volume reference to cover unculturable microorganisms in a large variety of habitats, which could not previously have been analyzed without metagenomic methodology. It features review articles as well as a large number of case studies, based largely on original publications and written by international experts. This second volume, Metagenomics in Different Habitats, covers such topics as: Viral genomes Metagenomics studies in a variety of habitats, including marine environments and lakes, soil, and human and animal digestive tracts Other habitats, including those involving microbiome diversity in human saliva and functional intestinal metagenomics; diversity of archaea in terrestrial hot springs; and microbial communities living at the surface of building stones Biodegradation Biocatalysts and natural products A special feature of this book is the highlighting of the databases and computer programs used in each study; they are listed along with their sites in order to facilitate the computer-assisted analysis of the vast amount of data generated by metagenomic studies. Such studies in a variety of habitats are described here, which present a large number of different system-dependent approaches in greatly differing habitats. Handbook of Molecular Microbial Ecology II is an invaluable reference for researchers in metagenomics, microbial ecology, microbiology, and environmental microbiology; those working on the Human Microbiome Project; microbial geneticists; and professionals in molecular microbiology and bioinformatics.

Enzymes in Food Processing

Since publication of the first edition of this book many new products have been commercially produced and the corresponding number of published papers has swollen.

Enzymes in Food Processing

Recent years have seen a rapid increase in the use of enzymes as food processing tools, as an understanding of their means of control has improved. Since publication of the first edition of this book many new products have been commercially produced and the corresponding number of published papers has swollen. This second edition has been fully revised and updated to cover changes in the last five years. It continues to provide food technologists, chemists, biochemists and microbiologists with an authoritative, practical and detailed review of the subject.