Extractive Bioconversions

Objectives for Extractive Bioconversion Bo Mattiasson and Olle Holst 2. Cultivation Using Membrane Filtration and Cell Recycling Olle Holst and Bo Mattiasson 3. Liquid-Liquid Extractive Membrane Reactors Jorge L. López, ...

Extractive Bioconversions

Contributors from European and US universities and corporations review recent developments in the integration of downstream processing and bioconversion, describing their experience with many separation technologies, including some still in the experimental stage. The topics include the construction

Process Integration in Biochemical Engineering

U. von Stockar Urs von Stockar, Luuk van der Wielen. Membrane-Assisted Extractive Bioconversions 1 Center for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais,1049-001 Lisboa, Portugal.

Process Integration in Biochemical Engineering

Process integration has been one of the most active research fields in Biochemical Engineering over the last decade and it will continue to be so if bioprocessing is to become more rational, efficient and productive. This volume outlines what has been achieved in recent years. Written by experts who have made important contributions to the European Science, Foundation Program on Process Integration in Biochemical Engineering, the volume focuses on the progress made and the major opportunities, and in addition on the limitations and the challenges in bioprocess integration that lie ahead. The concept of bioprocess integration is treated at various levels, including integration at the molecular, biological, bioreactor and plant levels, but also accounting for the integration of separation and mass transfer operations and biology, fluid dynamics and physiology, as well as basic science and process technology.

Separations Using Aqueous Phase Systems

EXTRACTIVE BIOCONVERSIONS IN AQUEOUS PHASE SYSTEMS Bo Mattiasson and Torbjörn G.I. Ling Department of Biotechnology, Chemical Center University of Lund, P. O. Box 124 S-221 00 Lund, Sweden INTRODUCTION The use of biocatalysts to perform ...

Separations Using Aqueous Phase Systems

The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.

Solvent Extraction

In such cases, there is a strong need for continuous extractive procedures during which the product is continuously removed. The integration of bioconversion and extraction is called extractive bioconversion. The large differences in ...

Solvent Extraction

The main challenge in modern solvent extraction separation is that most techniques are mainly empirical, specific and particular for narrow fields of practice and require a large degree of experimentation. This concise and modern book provides a complete overview of both solvent extraction separation techniques and the novel and unified competitive complexation/solvation theory. This novel and unified technique presented in the book provides a key for a preliminary quantitative prediction of suitable extraction systems without experimentation, thus saving researchers time and resources. Analyzes and compares both classical and new competitive models and techniques Offers a novel and unified competitive complexation / solvation theory that permits researchers to standardize some parameters, which decreases the need for experimentation at R&D Presents examples of applications in multiple disciplines such as chemical, biochemical, radiochemical, pharmaceutical and analytical separation Written by an outstanding scientist who is prolific in the field of separation science

Solvent Extraction Principles and Practice Revised and Expanded

In such cases , there is a strong need for continuous extractive procedures during which the product is continuously removed . The integration of bioconversion and extraction is called extractive bioconversion . The large differences in ...

Solvent Extraction Principles and Practice  Revised and Expanded

A complete and up-to-date presentation of the fundamental theoretical principles and many applications of solvent extraction, this enhanced Solvent Extraction Principles and Practice, Second Edition includes new coverage of the recent developments in solvent extraction processes, the use of solvent extraction in analytical applications and waste re

Enhancing Extraction Processes in the Food Industry

A number of extractive bioconversions relating to the conversion of complex compounds such as cellulose to simpler compounds such as alcohol, using ATPS were reported (Su and Feng 1999). Simultaneous extraction and purification of some ...

Enhancing Extraction Processes in the Food Industry

Extraction is an important operation in food engineering, enabling the recovery of valuable soluble components from raw materials. With increasing energy costs and environmental concerns, industry specialists are looking for improved techniques requiring less solvents and energy consumption. Enhancing Extraction Processes in the Food Industry is a comprehensive resource providing clear descriptions of the latest extraction methods and instruments used in food laboratories. The book begins with an overview of solvent extraction technology. It examines pulsed electric fields and their effect on food engineering, and the potential and limitations of microwave-assisted extraction. It explores diffusion processes and reviews what is known about electrical discharge processes in the extraction of biocompounds. Next, the book summarizes current knowledge on conventional and innovative techniques for the intensification of extractions from food and natural products, focusing on environmental impacts. It reviews recent developments in supercritical CO2 extraction of food and food products, describes the pressurized hot water extraction (PHWE) process, and examines future trends for PHWE. The book also examines essential oil extraction, and the tools and techniques of high pressure-assisted extraction. The authors demonstrate its application using litchi and longan fruits as examples. The final chapters focus on extrusion-assisted extraction, gas-assisted mechanical expression, mechanochemically assisted extraction, reverse micellar extraction, and aqueous two-phase extraction. The book concludes with a chapter on the treatment of soybeans through enzyme-assisted aqueous processing, examining the economics involved as well as the development of the process. A solid review of modern approaches that enhance extraction processes, this volume is destined to pave the way for future research and development in the field.

Wastewater Engineering Advanced Wastewater Treatment Systems

Extractive bioconversions with nonaqueous solvents, in: Mattiasson B, Holst O (Eds.), Extractive Bioconversions, ... Pertraction and membrane based solvent extraction of butyric acid in hollow fiber contactors, in: Proceedings of 14th ...

Wastewater Engineering  Advanced Wastewater Treatment Systems

As the global population grows and many developing countries modernize, the importance of water supply and wastewater treatment becomes a much greater factor in the welfare of nations. Clearly, in today’s world the competition for water resources coupled with the unfortunate commingling of wastewater discharges with freshwater supplies creates additional pressure on treatment systems. Recently, researchers focus on wastewater treatment by difference methods with minimal cost and maximum efficiency. This volume of the Wastewater Engineering: Advanced Wastewater Treatment Systems is a selection of topics related to physical-chemical and biological processes with an emphasis on their industrial applications. It gives an overview of various aspects in wastewater treatments methods including topics such as biological, bioremediation, electrochemical, membrane and physical-chemical applications. Experts in the area of environmental sciences from diverse institutions worldwide have contributed to this book, which should prove to be useful to students, teachers, and researchers in the disciplines of wastewater engineering, chemical engineering, environmental engineering, and biotechnology. We gratefully acknowledge the cooperation and support of all the contributing authors.

Biotechnology for Fuels and Chemicals

Strathman, H. and Gudematsch, W. (1991), in Extractive Bioconversions, Mattiasson, B. and Holst, O., eds., Marcel Dekker, New York, NY, pp. 67–89. 7. Boddeker, K. W. and Bengtson, G. (1991), in Pervaporation Membrane Separation ...

Biotechnology for Fuels and Chemicals

BRIAN H. DAVISON Oak Ridge National Laboratory MARK FINKELSTEIN National Renewable Energy Laboratory CHARLES E. WYMAN Oak Ridge National Laboratory The Eighteenth Symposium on Biotechnology for Fuels and Chemi cals continues to provide a forum for the presentation of research results and the exchange of ideas on advances in biotechnology for the produc tion of fuels and chemicals. Although the emphasis is on utilization of renewable resources, the scope of the Symposium is broader than this and includes bioconversion of fossil fuels and syngas and the new area of conversions in nonaqueous environments; these areas were discussed in Session 5 and in a Special Topic Discussion Group at the Symposium. In addition, recent developments in bioremediation were well represented in Session 6 and in the poster session. The Symposium involved both the development of new biological agents (such as enzymes or microbes) to carry out targeted conversions as well as bioprocess development. The first area covered improvements in enzymes as well as fundamental insights into substrate-enzyme inter actions and photosynthesis. The latter area focused on converting one material into another using biological agents through combinations of chemical engineering, biological sciences, and fermentation technology. This area also refers to an overall processing involving at least one bio logically catalyzed step in combination with other physical and/ or chemi cal processing operations. Agricultural crops, such as corn and corn fiber as well as woody biomass and lignocellulosic wastes, are emphasized for process feedstocks and their pretreatment investigated.

Bioconversion of Waste Materials to Industrial Products

Strathmann, H. and Gudernatsch, W. (1991) Continous removal of ethanol from bioreactor by pervaporation, in Extractive Bioconversions (eds B. Mattiasson and O. Holst), p. 328. Tanaka, M., Fukuri, M. and Matsuno, R. (1988) Removal of ...

Bioconversion of Waste Materials to Industrial Products

By covering both the general principles of bioconversion and the specific characteristics of the main groups of waste materials amenable to bioconversion methods, this new book provides the chemical, biochemical, agrochemical and process engineer with clear guidance on the use of these methods in devising a solution to the problem of industrial waste products.

Plant Cells

Even though their main application is bioseparation, increasing attention has been focused upon their use in extractive bioconversions or in situ extractions [75]. The integration of extraction and production in a bioreactor increases ...

Plant Cells

The recent achievements in engineering studies on plant cell cultures are reviewed, included are the gas concentration effects and bioprocess integration for the enhanced productivity of plant secondary metabolites. The metabolic engineering of plant secondary metabolite pathways and recombinant protein production from genetically modified plant cells are introduced. Large-scale plant micropropagation via somatic embryogenesis and hairy roots is discussed for efficient propagation of desease-free, genetically uniform and massive amounts of plants in vitro in massive amounts. Characterization and application of hairy plant roots endowed with photosynthetic functions is also covered in this special volume.

Aqueous Two phase Systems

ints, strate Top phase with product Bottom phase Product isolation unit Product Reaction vessel Settler unit matic presentation of extractive bioconversion in aqueous two-phase iocatalyst to initiate the reaction.

Aqueous Two phase Systems

In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Biotechnology 94

EXTRACTIVE BIOTRANSFORMATION - IMPLEMENTATION Work on fluidised bed adsorption has continued in Birmingham since 1986 (5). Particular emphasis has been placed upon the development of suitable solid phase compositions and geometries, ...

Biotechnology  94


Immobilized Cells and Organelles

In order to improve the extraction of inhibitory products in an aqueous twophase system , an increased top to bottom ... These prinicples can also be applied to extractive bioconversions in aqueous twophase systems which expands its ...

Immobilized Cells and Organelles

Cells and organelles are small units for biochemical synthetic purposes, often the smallest practically feasible unit since they contain coenzyme regenerating system, ordered enzyme sequences, etc.These volumes, besides giving some insight into basic technology (immobilisation procedures, etc.), also sum up the current know-how in this subject area and try to predict some future trends.The termimmobilized cells covers everything from dead cells with a single active enzyme species to cells proliferating on or within a three dimensional polymer matrix. The practical handling of these structures make them useful in various applications, e.g. large-scale production of biomolecules, biodegration, analysis, etc.

Integration of Membrane Processes Into Bioconversions

Decrease of the Extraction Rate , In : Proc . of Book of abstracts of the ISEC 99 , PD - 009 , 295 , Barcelona . 49. ... Liquid Extractive Membrane Reactors , In : B. Mattiasson and O. Holst ( Ed . ) , Extractive Bioconversions , p .

Integration of Membrane Processes Into Bioconversions

Contains papers from an August 1999 event held in Hungary, covering fundamentals of membrane processes, an introduction to biochemical engineering, and integration of membrane processes and bioconversions. Specific topics include transport phenomena in membrane separations, membrane based processes with immobilized interfaces, enzyme catalyzed reactions, bioreactor design using living cells or organisms, biocatalysts and membranes, and nanofiltration applications on food technology and environmental protection. Other subjects are membrane bioreactors, extraction of aromas from active fermentation reactors by pervaporation, and membrane fermentors. The editor is affiliated with the Research Institute for Chemical and Process Engineering. Annotation copyrighted by Book News, Inc., Portland, OR

Partitioning In Aqueous Two Phase System

This extraction technology appears to be especially well suited for the first steps of an isolation procedure, ... is their use as carrier systems in extractive bioconversions, which appears to be of increasing interest at present.

Partitioning In Aqueous Two     Phase System

Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Sustainable Green Chemical Processes and their Allied Applications

The simultaneous mechanical cell disruption and extraction of intracellular proteins from yeasts has been successfully ... Also, extractive bioconversions using enzymes and whole cells as biocatalysts have been performed in ATPSs.

Sustainable Green Chemical Processes and their Allied Applications

Urbanization, industrialization, and unethical agricultural practices have considerably negative effects on the environment, flora, fauna, and the health and safety of humanity. Over the last decade, green chemistry research has focused on discovering and utilizing safer, more environmentally friendly processes to synthesize products like organic compounds, inorganic compounds, medicines, proteins, enzymes, and food supplements. These green processes exist in other interdisciplinary fields of science and technology, like chemistry, physics, biology, and biotechnology, Still the majority of processes in these fields use and generate toxic raw materials, resulting in techniques and byproducts which damage the environment. Green chemistry principles, alternatively, consider preventing waste generation altogether, the atom economy, using less toxic raw materials and solvents, and opting for reducing environmentally damaging byproducts through energy efficiency. Green chemistry is, therefore, the most important field relating to the sustainable development of resources without harmfully impacting the environment. This book provides in-depth research on the use of green chemistry principles for a number of applications.

Bioseparations of Proteins

Hatton, T.A. (1989) Reverse Micelle Extraction of Proteins. In Surfactant-Based Separation Processes, ... In Extractive Bioconversions, Mattiasson, B., and O. Holst, O., Eds., Marcel Dekker: New York, pp. 173–178. Kauzmann, W. (1959).

Bioseparations of Proteins

This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation. Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations. Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures. Key Features * Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include: * detailed data on biological stability under various bioseparation conditions * extensive case studies from the literature on separation processes, validation, and economics * simplified analysis of protein refolding and inactivation mechanisms * consideration of adsorption theories and the effect of heterogeneity * coverage of both classical and novel bioseparation techniques, including chromatographic procedures

Aqueous Two Phase Systems

Section VI Aqueous Two - Phase Systems in Large - Scale Processes : Biotechnology A. Extractive Bioconversions Articles 52 through 54 B. Proteins : Downstream Processing , etc. Articles 55 through 61 C. Design of Proteins for Enhanced ...

Aqueous Two Phase Systems

This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features * Use of affinity partitioning for enzyme purification * Separation and study of cells * Isolation of plasma membranes * Large scale biotechnical use

Biotechnology Potentials and Limitations

EXTRACTIVE BIOCONVERSIONS The efficiency of a bioconversion can be bettered either by increasing the concentration of the starting reagents or by reducing those of the products in the reaction mixture. In extractive fermentations this ...

Biotechnology  Potentials and Limitations

hurdle will be in the latter area. The technological hurdles will be formi dable but will not limit what happens: once the basic ideas are available, the technology will be developed. The unique part of biotechnology will be to imagine what the possibilities are. There was a discussion in several of the groups on the problems of intro ducing a novel science into a social and economic context. What biotech nologists are learning on this matter is not novel, although that does not make it any less important or difficult. People in the development of elec tronics and computers, in the pharmaceutical industry, and in many other types of industry that have grown from university research have had to face these problems in the past. It is the old situation of having to reinvent the wheel again and again. There is one aspect on which biotechnology seems to have handled this inherent difficulty better than some of our predecessor technologies: the people in the biotechnology companies by and large take a rather academic approach to free communication with one another at meetings such as this and open publication of many of their basic findings in the literature. This seems unique and certainly is different from the experience of the recent Silicone Valley Industry, which in other ways tries to emulate an academic environment, but not in open and free publication.

Encyclopedia of Agricultural Food and Biological Engineering Print

1 Flow diagram showing the extractor , separator , and solvent recovery process for extraction of oil from soybeans . ... Mattiason , B .; Holste , 0 .; Eds . Extractive Bioconversions ; Marcel Dekker : New York , 1991 . 10.

Encyclopedia of Agricultural  Food  and Biological Engineering  Print

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